Translational Studies in Malaria

Heterogeneity of the human immune response to malaria infection and vaccination driven by latent cytomegalovirus infection

Reena Mukhiya, Wim A Fleischmann, Jessica R Loughland, Jo-Anne Chan, Fabian de Labastida Rivera, Dean Andrew, James G Beeson, James S McCarthy, Bridget E Barber, J Alejandro Lopez, Christian Engwerda, Richard Thomson-Luque, Michelle J Boyle

2024-11-01

BACKGROUND: Human immune responses to infection and vaccination are heterogenous, driven by multiple factors including genetics, e..

Author Correction: The transcriptome of circulating sexually committed Plasmodium falciparum ring stage parasites forecasts malaria transmission potential (Nature Communications, (2020), 11, 1, (6159), 10.1038/s41467-020-19988-z)

SK Prajapati, R Ayanful-Torgby, Z Pava, MC Barbeau, FK Acquah, E Cudjoe, C Kakaney, JA Amponsah, E Obboh, AE Ahmed, BK Abuaku, JS McCarthy, LE Amoah, KC Williamson

2022-12-01

The original version of this Article contained an error in Supplementary Table 3 giving details on used primers and probes. The co..

Positron emission tomography and magnetic resonance imaging of the brain in experimental human malaria, a prospective cohort study

J Woodford, A Gillman, P Jenvey, J Roberts, S Woolley, BE Barber, M Fernandez, S Rose, P Thomas, NM Anstey, JS McCarthy

2022-12-01

Cerebral malaria is the most serious manifestation of severe falciparum malaria. Sequestration of infected red blood cells and mic..

The in-vivo dynamics of Plasmodium falciparum HRP2: implications for the use of rapid diagnostic tests in malaria elimination

L Marquart, L Webb, P O’Rourke, ML Gatton, MS Hsiang, M Kalnoky, IK Jang, H Ntuku, DR Mumbengegwi, GJ Domingo, JS McCarthy, S Britton

2022-12-01

Background: Rapid diagnostic tests (RDTs) that rely on the detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) ha..

Analytical validation of a real-time hydrolysis probe PCR assay for quantifying Plasmodium falciparum parasites in experimentally infected human adults

CYT Wang, EL Ballard, Z Pava, L Marquart, J Gaydon, SC Murphy, D Whiley, P O’Rourke, JS McCarthy

2021-12-01

Background: Volunteer infection studies have become a standard model for evaluating drug efficacy against Plasmodium infections. M..

Experimental human hookworm infection: a narrative historical review

PR Chapman, P Giacomin, A Loukas, JS McCarthy

2021-12-01

AUIn 1896,: Pleaseconfirmthatallheadinglevelsarerepresentedcorrectly: a serendipitous laboratory accident led to the understanding..

Reduced circulating dendritic cells in acute Plasmodium knowlesi and Plasmodium falciparum malaria despite elevated plasma Flt3 ligand levels

JR Loughland, T Woodberry, D Oyong, KA Piera, FH Amante, BE Barber, MJ Grigg, T William, CR Engwerda, NM Anstey, JS McCarthy, MJ Boyle, G Minigo

2021-12-01

Background: Plasmodium falciparum malaria increases plasma levels of the cytokine Fms-like tyrosine kinase 3 ligand (Flt3L), a hae..

Vaccination of human participants with attenuated Necator americanus hookworm larvae and human challenge in Australia: a dose-finding study and randomised, placebo-controlled, phase 1 trial

PR Chapman, R Webster, P Giacomin, S Llewellyn, L Becker, MS Pearson, F De Labastida Rivera, P O'Rourke, CR Engwerda, A Loukas, JS McCarthy

2021-12-01

Background: Control of human hookworm infection would be greatly aided by the development of an effective vaccine. We aimed to dev..

Reply to White and Watson

M Rebelo, JS McCarthy, DS Khoury

2021-08-15

Parasite Viability as a Superior Measure of Antimalarial Drug Activity in Humans

M Rebelo, R Pawliw, J Gower, L Webb, H Mitchell, Z Pava, RE Watts, MP Davenport, JS McCarthy, DS Khoury

2021-06-15

Background: Artemisinin derivatives are the leading class of antimalarial drugs due to their rapid onset of action and rapid clear..

Positron emission tomography and magnetic resonance imaging in experimental human malaria to identify organ-specific changes in morphology and glucose metabolism: A prospective cohort study

J Woodford, A Gillman, P Jenvey, J Roberts, S Woolley, BE Barber, M Fernandez, S Rose, P Thomas, NM Anstey, JS McCarthy

2021-05-01

Background Plasmodium vivax has been proposed to infect and replicate in the human spleen and bone marrow. Compared to Plasmodium ..

Th2-like T Follicular Helper Cells Promote Functional Antibody Production during Plasmodium falciparum Infection

JA Chan, JR Loughland, F de Labastida Rivera, A SheelaNair, DW Andrew, NL Dooley, BD Wines, FH Amante, L Webb, PM Hogarth, JS McCarthy, JG Beeson, CR Engwerda, MJ Boyle

2020-12-22

CD4⁺ T follicular helper cells (Tfh) are key drivers of antibody development. During Plasmodium falciparum malaria in c..

The transcriptome of circulating sexually committed Plasmodium falciparum ring stage parasites forecasts malaria transmission potential

Surendra K Prajapati, Ruth Ayanful-Torgby, Zuleima Pava, Michelle C Barbeau, Festus K Acquah, Elizabeth Cudjoe, Courage Kakaney, Jones A Amponsah, Evans Obboh, Anwar E Ahmed, Benjamin K Abuaku, James S McCarthy, Linda E Amoah, Kim C Williamson

2020-12-02

Malaria is spread by the transmission of sexual stage parasites, called gametocytes. However, with Plasmodium falciparum, gametocy..

Liver Function Test Abnormalities in Experimental and Clinical Plasmodium vivax Infection

Anand Odedra, Lachlan Webb, Louise Marquart, Laurence J Britton, Stephan Chalon, Joerg J Moehrle, Nicholas M Anstey, Timothy William, Matthew J Grigg, David G Lalloo, Bridget E Barber, James S McCarthy

2020-11-01

Liver transaminase elevations after treatment in malaria volunteer infection studies (VISs) have raised safety concerns. We invest..

Safety, tolerability, pharmacokinetics, and antimalarial efficacy of a novel Plasmodium falciparum ATP4 inhibitor SJ733: a first-in-human and induced blood-stage malaria phase 1a/b trial

Aditya H Gaur, James S McCarthy, John C Panetta, Ronald H Dallas, John Woodford, Li Tang, Amber M Smith, Tracy B Stewart, Kristen C Branum, Burgess B Freeman, Nehali D Patel, Elizabeth John, Stephan Chalon, Shelley Ost, Ryan N Heine, Julie L Richardson, Robbin Christensen, Patricia M Flynn, Yvonne Van Gessel, Branko Mitasev

2020-08-01

Background: (+)-SJ000557733 (SJ733) is a novel, orally bioavailable inhibitor of Plasmodium falciparum ATP4. In this first-in-huma..

Assays for quantification of male and female gametocytes in human blood by qRT-PCR in the absence of pure sex-specific gametocyte standards

Claire YT Wang, Emma Ballard, Stacey Llewellyn, Louise Marquart, Teun Bousema, James S McCarthy, Katharine A Collins

2020-06-23

Background Malaria transmission from humans to Anopheles mosquitoes requires the presence of gametocytes in human peripheral circu..

A Plasmodium vivax experimental human infection model for evaluating efficacy of interventions

Katharine A Collins, Claire YT Wang, Matthew Adams, Hayley Mitchell, Greg J Robinson, Melanie Rampton, Suzanne Elliott, Anand Odedra, David Khoury, Emma Ballard, Todd B Shelper, Leonardo Lucantoni, Vicky M Avery, Stephan Chalon, Joerg J Moehrle, James S McCarthy

2020-06-01

BACKGROUND. Interventions that interrupt Plasmodium vivax transmission or eliminate dormant P. vivax liver-stage parasites will be..

Infection-induced plasmablasts are a nutrient sink that impairs humoral immunity to malaria

Rahul Vijay, Jenna J Guthmiller, Alexandria J Sturtz, Fionna A Surette, Kai J Rogers, Ramakrishna R Sompallae, Fengyin Li, Rosemary L Pope, Jo-Anne Chan, Fabian de Labastida Rivera, Dean Andrew, Lachlan Webb, Wendy J Maury, Hai-Hui Xue, Christian R Engwerda, James S McCarthy, Michelle J Boyle, Noah S Butler

2020-05-18

Plasmodium parasite–specific antibodies are critical for protection against malaria, yet the development of long-lived and effecti..

Early Endothelial Activation Precedes Glycocalyx Degradation and Microvascular Dysfunction in Experimentally Induced Plasmodium falciparum and Plasmodium vivax Infection

John Woodford, Tsin W Yeo, Kim A Piera, Kristy Butler, J Brice Weinberg, James S McCarthy, Nicholas M Anstey, Bridget E Barber

2020-05-01

Endothelial activation and microvascular dysfunction are key pathogenic processes in severe malaria. We evaluated the early role o..

Transcriptional profiling and immunophenotyping show sustained activation of blood monocytes in subpatentPlasmodium falciparuminfection

Jessica R Loughland, Tonia Woodberry, Matt Field, Dean W Andrew, Arya SheelaNair, Nicholas L Dooley, Kim A Piera, Fiona H Amante, Enny Kenangalem, Ric N Price, Christian R Engwerda, Nicholas M Anstey, James S McCarthy, Michelle J Boyle, Gabriela Minigo

2020-01-01

Objectives Malaria, caused by Plasmodium infection, remains a major global health problem. Monocytes are integral to the immune re..

Loss of complement regulatory proteins on red blood cells in mild malarial anaemia and in Plasmodium falciparum induced blood-stage infection

DA Oyong, JR Loughland, A Sheelanair, D Andrew, FDL Rivera, KA Piera, T William, MJ Grigg, BE Barber, A Haque, CR Engwerda, JS McCarthy, NM Anstey, MJ Boyle

2019-09-18

Background: Anaemia is a major consequence of malaria, caused by the removal of both infected and uninfected red blood cells (RBCs..

Human challenge models: tools to accelerate the development of malaria vaccines

MM Cooper, C Loiseau, JS McCarthy, DL Doolan

2019-03-04

Introduction: Malaria challenge models, where healthy human volunteers are intentionally infected with Plasmodium species parasite..

Assessing Plasmodium falciparum transmission in mosquito-feeding assays using quantitative PCR

CYT Wang, JS McCarthy, WJ Stone, T Bousema, KA Collins, S Bialasiewicz

2018-07-05

Background: Evaluating the efficacy of transmission-blocking interventions relies on mosquito-feeding assays, with transmission ty..

A controlled human malaria infection model enabling evaluation of transmission-blocking interventions

KA Collins, CYT Wang, M Adams, H Mitchell, M Rampton, S Elliott, IJ Reuling, T Bousema, R Sauerwein, S Chalon, JJ Möhrle, JS McCarthy

2018-04-02

BACKGROUND. Drugs and vaccines that can interrupt the transmission of Plasmodium falciparum will be important for malaria control ..